Identification of differentially expressed proteins at four growing stages in chicken liver

Asian - Australasian Journal of Animal Sciences, Oct, 2008 by K.Y. Lee, K.C. Jung, B.G. Jang, K.D. Choi, J.T. Jeon, J.H. Lee

Kanai, M., T. Soji, D. C. Herbert. 1997. Biogenesis and function of lipolysosomes in developing chick hepatocytes. Microsc. Res. Tech. 39:444-452.

Kelly, V. C., S. Kuy, D. J. Palmer, Z. Xu, S. R. Davis and G. J. Cooper. 2006. Characterization of bovine seminal plasma by proteomics. Proteomics 6:5826-5833.

Kim, N. K., J. H. Lim, M. J. Song, O. H. Kim, B. Y. Park, M. J. Kim, I. H. Hwang and C. S. Lee. 2007. Developmental Proteomic Profiling of Porcine Skeletal Muscle during Postnatal Development. Asian-Aust. J. Anim. Sci. 20:1612-1617.

Kuhajda, F. P. 2000. Fatty-acid synthase and human cancer:New perspectives on its role in tumor biology. Nutr. 16:202-208.

Naaby-Hansen, S., M. D. Waterfield and R. Cramer. 2001. Proteomics-post-genomic cartography to understand gene function. Trends Pharmacol. Sci. 22:376-384.

Pandey, A. and M. Mann. 2000. Proteomics to study genes and genomes. Nature 405:837-846.

Park, Y. D., S. Y. Kim, H. S. Jang, E. Y. Seo, J. H. Namkung, H. S. Park, S. Y. Cho, Y. K. Paik and J. M. Yang. 2004. Towards a proteomic analysis of atopic dermatitis: A two-dimensional-polyacrylamide gel electrophoresis/mass spectrometric analysis of cultured patient-derived fibroblasts. Proteomics 4:3446-3455.

Smith, S. 1994. The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. FASNEB J. 8:1248-1259.

Smith, S., A. Witkowski and A. K. Joshi. 2003. Structural and functional organization of the animal fatty acid synthase. Prog. Lipid Res. 42:289-317.

Stern, C. D. 2004. The chick embryo - Past, present and future as a model system in developmental biology. Mech. Dev. 121:1011-1013.

Stern, C. D. 2005. The chick: A great model system becomes even greater. Dev. Cell 8:9-17.

Witkowski, A., V. S. Rangan, Z. I. Randhawa, C. M. Amy and S. Smith. 1991. Structural organization of the multifunctional animal fatty acid synthase. Eur. J. Biochem. 198:571-579.

K. Y. Lee, K. C. Jung, B. G. Jang (1), K. D. Choi (2), J. T. Jeon (3) and J. H. Lee *

Division of Animal Science and Resources, College of Agriculture and Life Sciences Chungnam National University, Daejeon 305-764, Korea

(1) Livestock Research Center, National Institute of Subtropical Agriculture, RDA, Jeju 690-150, Korea.

(2) The Graduate School of Bio & Information Technology, Hankyong National University, Ansung 456-749, Korea.

(3) Division of Applied Life Science, Gyeongsang National University, 900 Gajwa-dong, Jinju, Gyeongnam 660-701, Korea.

* Corresponding Author: J. H. Lee. Tel: 82-42-821-5779, Fax: 82-42-825-9754, E-mail: junheon@cnu.ac.kr

Table 1. The identification results for the differentially expressed
proteins in four growing stages in chicken liver

Spot ID     Accession No     Protein information              %

       1    -                -                                -
       2    gi|45383974      albumin                          39
       3    gi|50752703      similar to Sorbitol              52
                             dehydrogenase
       4    gi|62738642      Chicken Annexin V                39
       5    XP_421496.1      similar to catalase              31
       6    gi|1345958       Fatty acid synthase               7
       7    XP_421496.1      similar to catalase              30
       8    gi|57530355      malate dehydrogenase 1           34
       9    gi|15420977      malic enzyme                     30
      10    gi|57524986      heat shock 70 kDa protein 9B     17
      11    XP_421496.1      similar to catalase              26
      12    gi|230360        Triose phosphate isomerase       38
      13    gi|46048768      Enolase 1                        24

                                      Highest
Spot ID         pI           kDa     expression (1)

       1         -            -           0
       2        5.51         69.872      32
       3        7.09         38.12       32
       4        5.61         36.045      32
       5        7.3          55.77       32
       6        5.94        274.607      32
       7        7.3          55.77       32
       8        6.92         36.52       32
       9        6.45         61.961      32
      10        6.09         73.141       0
      11        7.3          55.77        0
      12        7.26         26.527       0
      13        6.17         47.275       0

Note that two proteins (spot No. 6, 9) were identified as lipid related
proteins.

(1) Observed highest expression among four growing stages
(0, 10, 21, 32 weeks of age).