The expression patterns of Cdc25A, Cdc25B, Sox2 and Mnb in central nervous system in early chicken embryos

Asian - Australasian Journal of Animal Sciences, June, 2009 by Hui Zhang, Junhui Qin, Jingjing Cao, Nainan Hei, Chunsheng Xu, Ping Yang, Haili Liu, Xiaohong Chu, Huijun Bao, Qiusheng Chen

In summary, we produced RNA probes of four genes and detected transcription level by whole mount in situ hybridization in the same developmental stage of the CNS in chick embryos. In general, all four gene expression patterns were similar in CNS, only having some distinction in specific region and in concentration. The results suggest that chicken Cdc25A, Cdc25B, Sox2 and Mnb expression patterns support specific roles of these regulators in CNS development because of gene expression with a spatiotemporal pattern in embryonic development. The overlap in expression in the same developmental stage of the CNS suggests that the four genes may be functional similar or have a regulatory relationship in CNS development (Collignon et al., 1996). The mechanisms of regulating CNS development indicate that the four genes are involved in cell cycle progress and cell proliferation and differentiation events. Moreover, the four genes may regulate each other coordinately. However, the gene network of regulating CNS development is precise and complicated. The development of the CNS of the chick requires a precise and reproducible pattern of neuroblast proliferation during embryonic neurogenesis. Here, we elucidated the mechanisms only in a morphologic manner. Further investigation is needed to identify molecules involved in CNS development and to elucidate the mechanisms of CNS development.

ACKNOWLEDGEMENTS

We would like to thank Fabienne Pituello from CNRS for helping us to design primers.

Received September 12, 2008; Accepted January 19, 2009

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