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Industry: Email Alert RSS FeedDevelopment of a Dual-Luciferase Reporter System for In Vivo Visualization of MicroRNA Biogenesis and Posttranscriptional Regulation
Journal of Nuclear Medicine, The, February, 2008 by Chung, June-Key; Hwang, Do Won; Jeong, Jae Min; Kim, Soonhag; Lee, Dong Soo; Lee, Myung Chul; Lee, Ji Young
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MicroRNAs (miRNAs) function in mammalian cells via translational repression or messenger RNA (mRNA) cleavage of target genes by base-pairing with 3' untranslated regions (UTRs) of target mRNAs. Although miRNAs are involved in cell differentiation or organ development, posttranscritptional regulation of miRNA is not well understood.
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Here, we developed a dual-luciferase reporter system for monitoring in vivo endogenous transcription of primary miRNA (pri-miRNA) and also the mature miRNA activity simultaneously. Methods: miR23P639/Fluc plasmid carrying firefly luciferase (Fluc) under the control ...
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