Molecular Marker Mapping of RSV4, a Gene Conferring Resistance to all Known Strains of Soybean Mosaic Virus.

Crop Science, September, 2000 by Buss, Glenn R.; Hayes, Alec J.; Ma, Guorong; Maroof, M.A. Saghai

Soybean mosaic virus (SMV) is a prevalent viral pathogen of soybean [Glycine max (L.) Merr.] wherever it is grown. Several genes that confer resistance in soybean to soybean mosaic virus have been identified. One of these resistance loci, Rsv4, confers resistance to all the known strain groups of SMV. This study was conducted to determine the map position of the Rsv4 locus in the soybean genome. A population of 255 [F.sub.2] individuals from the cross of the SMV resistant line LR2 (Rsv4) by the susceptible line Lee68 (rsv4) was evaluated in a mapping study. DNA from 12 to 15 [F.sub.2] individuals being either homozygous resistant or susceptible were pooled to produce bulk resistant and bulk susceptible DNA samples. Parents and bulks were screened with 101 AFLP primer pairs and two...

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