Molecular marker analysis of seed size in soybean. (Crop Breeding, Genetics & Cytology).

Crop Science, January, 2003 by Cianzio, Silvia R.; Fehr, Walter R.; Hoeck, Joseph A.; Johnson, Susan L.; Shoemaker, Randy C.; Welke, Grace A.

Seed size is an important attribute of soybean [Glycine max (L.) Merr.] for some food uses. The objectives of this study were to identify simple-sequence-repeat (SSR) markers associated with quantitative trait loci for seed size (SSQTL) and to compare the effectiveness of phenotypic selection and marker-assisted selection for seed size among individual [F.sub.2] plants. Three small-seeded lines were crossed to parents with normal seed size to form three two-parent populations. The parents of the populations were screened with 178 SSR markers to identify polymorphism. Population 1 (Pop 1) had 75 polymorphic SSR markers covering 1306 centimorgans (cM), Pop 2 had 70 covering 1143 cM, and Pop 3 had 82 covering 1237 cM. Seed size of each population was determined with 100 [F.sub.2]...

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