A cleaved amplified polymorphic sequence (CAPS) marker associated with root-knot nematode resistance in sugarbeet.(Genomics, Molecular Genetics & Biotechnology)

Crop Science, September, 2003 by Weiland, J.J.; Yu, M.H.

Resistance to root-knot nematode (Meloidogyne spp.) previous was introgressed into sugar beet (Beta vulgaris L.) from wild beet [B. vulgaris ssp. maritima (L.) Arcang] and was demonstrated to be dominant and simply inherited. Since resistance conferred by this gene was effective against six different Meloidogyne spp. tested, the locus was designated R6m-1. An interpollinated progeny population of resistant heterozygotes segregating for R6m-1, was exposed to nematodes in a greenhouse and rated for root knot disease symptoms. Resistance vs. susceptibility segregated at approximately a 4:1 ratio and 120 resistant roots and 48 susceptible roots were chosen for the generation of molecular markers linked to the resistance trait. Bulked DNA samples prepared from shoots sprouting from the...

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