Genetic relationships among smooth bromegrass cultivars of different ecotypes detected by AFLP markers.(Genomics, Molecular Genetics & Biotechnology)

Crop Science, January, 2004 by Coulman, Bruce E.; Ferdinandez, Yasas S.N.

Smooth bromegrass (Bromus inermis Leyss.), the most commonly cultivated perennial bromegrass species in North America, belong to three ecotypes; northern, intermediate, and southern. The objective of this study was to analyze the genetic relationships of cultivars belonging to these ecotypes by amplified fragment length polymorphic (AFLP) markers. Seven AFLP primer combinations produced 176 polymorphic markers, which occurred in different frequencies in different cultivars, with no ecotype or cultivar specific markers. Cluster analysis and principle component analysis grouped the cultivars according to their pedigrees rather than by ecotypes. The southern cultivars (Baylor, Lincoln, Beacon, and Blair), older cultivars developed in Iowa and Nebraska, grouped distantly to the rest...

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