Mapping late blight resistance in Solanum microdontum Bitter.(Genomics, Molecular Genetics & Biotechnology)

Crop Science, January, 2005 by Bisognin, D.A.; Bryan, G.; Buszka, L.; Douches, D.S.; Wang, D.

A diploid Solanum population was developed with the objective of mapping quantitative trait loci (QTL) associated with resistance to Phytophthora infestans (Mont.) de Bary, the causal organism of late blight. The mapping population was a cross between a late blight resistant selection of Solanum microdontum Bitter and a susceptible diploid breeding clone. The progeny of 109 clones and the parents were field tested for foliar late blight reaction in 1999 and 2000 and for vine maturity in 2000 and 2001. Parents and progeny were genotyped with isozymes and simple sequence repeats (SSR) markers. A total of 161 pairs of SSR primers were screened, from which 74 amplified polymorphic bands in Metaphor agarose or polyacrylamide gels yielding a total of 109 SSR loci. The isozyme and SSR...

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