Hazardous effects of effluent from the chrome plating industry: 70 kDa heat shock protein expression as a marker of cellular damage in transgenic Drosophila melanogaster - hsp70-lacZ - Research

Environmental Health Perspectives, Dec, 2003 by Indranil Mukhopadhyay, Daya Krishna Saxena, Debapratim Kar Chowdhuri

Table 1. Metal analysis of effluent from the
chrome plating industry.

Metals present   Concentration ([micro]g/mL)

Cadmium                      ND
Chromium                    230.3
Zinc                        204.5
Iron                        175.9
Nickel                      120.8
Manganese                    43.6
Lead                         23.0
Copper                       83.6

ND, not detected.

Table 2. Effect of effluent from chrome plating industry on
reproduction in D. melanogaster(hsp70-lacZ)[Bg.sup.9].

                                      Total          Eggs laid per
                                    fecundity      female in 10 days
Group                             (no, of eggs)    (mean [  or -] SE)

Control                               2,350       235.0 [  or -] 8.90
0.05 [micro]L/mL
  Treated male x treated female       2,330       233.0 [  or -] 7.6
  Treated male x normal female        2,329       232.9 [  or -] 8.1
  Normal male x treated female        2,345       234.5 [  or -] 7.7
0.1 [micro]L/mL
  Treated male x treated female       2,328       232.8 [  or -] 7.2
  Treated male x normal female        2,327       232.7 [  or -] 7.3
  Normal male x treated female        2,331       233.1 [  or -] 7.3
0.1 [micro]L/mL
  Treated male x treated female       2,319       231.9 [  or -] 8.2
  Treated male x normal female        2,322       232.2 [  or -] 7.9
  Normal male x treated female        2,329       232.9 [  or -] 7.7
0.1 [micro]L/mL
  Treated male x treated female       1,990       199.0 [  or -] 8.6 *
  Treated male x normal female        1,998       199.8 [  or -] 8.3 *
  Normal male x treated female        2,318       231.8 [  or -] 7.3

                                                      Reproductive
Group                             Fertility (%)       performance

Control                               95.6        224.7 [  or -] 7.6
0.05 [micro]L/mL
  Treated male x treated female       93.3        217.4 [  or -] 6.9
  Treated male x normal female        91.1        212.2 [  or -] 7.1
  Normal male x treated female        94.0        220.4 [  or -] 7.0
0.1 [micro]L/mL
  Treated male x treated female       92.8        216.0 [  or -] 7.2
  Treated male x normal female        92.0        214.1 [  or -] 7.6
  Normal male x treated female        93.0        216.8 [  or -] 7.3
0.1 [micro]L/mL
  Treated male x treated female       93.3        216.4 [  or -] 6.8
  Treated male x normal female        93.6        217.3 [  or -] 7.6
  Normal male x treated female        91.1        212.2 [  or -] 7.3
0.1 [micro]L/mL
  Treated male x treated female       86.6        172.3 [  or -] 8.1 *
  Treated male x normal female        88.4        176.6 [  or -] 7.8 *
  Normal male x treated female        91.3        211.6 [  or -] 7.8

* p < 0.05 versus control.

We thank the director of the ITRC for his keen interest throughout the study. We thank S.C. Lakhotia (Cytogenetics Laboratory, Banaras Hindu University, Varanasi) for [Bg.sup.9] fly strain; S. Lindquist (Whitehead Institute, Cambridge, Massachusetts, USA) for Drosophila Hsp70 monoclonal antibody 7Fb; V.K. Bajpai (Electron Microscopy Unit, Central Drug Research Institute, Lucknow, India) for electron microscopy; and N. Narayanan for X-gal staining.