Using Laser Microdissection And Representation- Difference Analysis To Isolate Novel Prostate Cancer Tumor Genes In African-American And Caucasian Men

Life Sciences & Biotechnology Update, Feb, 2001

This Johns Hopkins University) research has made significant progress on all three of its specific aims: (1) obtaining metastatic prostate cancer DNA of quality sufficient for representational difference analysis (RDA), which is obtained using alternate means; (2) employing RDA to identify DNA regions which have been homozygously deleted, which has been achieved with identification of a novel region of homozygous deletion on chromosome 12p; (3) prioritizing regions of homozygous deletion based on frequency of deletion of this region in metastatic tumors, and identification of candidate genes within these regions, which has been achieved through previously unidentified deletion of the identified chromosome 12p region in 50% of metastatic prostate cancers studied. Examination of genes within this region suggests that p27 or TEL appear to be likely candidates. The results validate this approach, and additional studies are underway to identify additional genomic regions of interest in metastatic prostate cancer.

(Order this LIFE SCIENCES & BIOTECHNOLOGY UPDATE reviewed report from InfoTeam Inc., P.O. Box 15640, Plantation, FL 33318-5640; Phone (954) 473-9560, Fax (954) 473-0544: Report No. L20010217; 1999, 18 pp. Price: $89.00, prepaid.)