Bacteria bound to cloth; glucoprotamin; toluidine blue O; surgical helmets versus filtering masks

AORN Journal, August, 2004 by George Allen

Cloth as a transmitter of infectious agents

American Journal of Infection Control

February 2004

Protecting patients from health care-associated infections is a multifaceted process rooted in the concept that these infections may be caused by pathogens from other patients or hospital personnel. Although health care workers' hands are the primary mechanism of transmission, their clothing also easily can become contaminated and serve as a source of infection transmission. The purpose of this study was to analyze the bacteria-binding properties of different cloth materials. (1)

Cloth fibers (ie, cotton, nylon, polyester, acrylic, wool) were incubated with bacterial suspensions of standard and clinically isolated strains of Staphylococcus aureus and Pseudomonas aeruginosa in silicone-coated tubes. Colony-forming units (CFUs) were determined, and the binding ratios were calculated. Statistical methods, including means and standard deviations, were used to analyze the data and compare observed differences.

Findings. Staphylococcus aureus strains bound to acrylic, polyester, and wool fibers at very high levels (ie, on average, 87.6%, 96.2%, and 63.2%, respectively). Pseudomonas aeruginosa strains also bound to acrylic, polyester, and wool at high levels (ie, on average, 95.4%, 99.9%, and 84.7%, respectively). Staphylococcus aureus bound to cotton and nylon fibers at very low levels (ie, 2% and 0.9%, respectively), and Pseudomonas aeruginosa strains bound to cotton and nylon fibers at 15% and 8%, respectively.

Clinical implications. The results of this study suggest that polyester, acrylic, and wool clothing can be good carriers of Staphylococcus aureus and Pseudomonas aeruginosa strains, two major pathogens commonly involved in health care-associated infections. Perioperative nurses and managers should consider these findings when evaluating scrubs or warm-up jackets to be used in perioperative settings.

Performance of glucoprotamin as a high-level disinfectant

Infection Control and Hospital Epidemiology

October 2003

Endoscopes and other heat-labile, semicritical instruments must undergo high-level disinfection or sterilization before reuse. Glucoprotamin is a new disinfecting agent licensed in Europe as a high-level disinfectant for instruments. The purpose of this prospective, observational study was to determine the efficacy of glucoprotamin trader clinical conditions, such as reprocessing used instruments in a hospital setting. (2)

Used specula and forceps were collected during 10 consecutive days and sent to a laboratory within four hours of having been used. Without their having been washed or brushed, the instruments were immersed in saline solution. A 1.5% concentration of glucoprotamin was added, and the instruments were soaked for 60 minutes. Biocidal activity was assessed by determining the difference in CFU on instruments before and after disinfection.

Findings. One hundred thirty-seven instruments (ie, 73 specula, 64 forceps) were studied. Before processing with glucoprotamin, the average bioburden per instrument was between [10.sup.5] CFU and [10.sup.7] CFU. After processing, no vegetative bacteria were isolated in any sample. Bacterial killing was more than 5 [log.sub.10] CFU (range 5.01 to 7.17).

Clinical implications. This clinical study showed that glucoprotamin was effective as a high-level disinfectant for reprocessing instruments. Bacterial killing in excess of 5 [log.sub.10] CFU was observed after one hour even though the instruments did not undergo a cleaning process before they were submerged in the disinfectant. This product must be registered and approved by the Environmental Protection Agency before it can be marketed in the United States as a product for high-level disinfection. If this product is approved, it will represent an additional choice for perioperative nurses for reprocessing instruments and supplies that require high-level disinfection.

Light-induced killing of bacteria

Infection Control and Hospital Epidemiology

October 2003

Instruments, equipment, and surfaces can harbor microorganisms and serve as a source of infecting pathogens for both patients and health care personnel. Keeping the environment in the perioperative setting as clean as possible through routine damp dusting and cleaning with a hospital-grade disinfectant is a daunting task, and the level of success depends on the skill and diligence of the support personnel assigned to this task. The purpose of this study was to determine whether light-induced killing of bacteria could be achieved when a photosensitizer was incorporated into material that could be used to coat a surface. (3)

The bottom surfaces of glass containers were coated with a cellulose acetate layer containing the photosensitizer toluidine blue O. Cellulose acetate coatings without toluidine blue O were used as controls. Suspensions of a strain of methicillin-resistant Staphylococcus aureus (MRSA), a gram-positive pathogen, and a clinical isolate of Pseudomonas aeruginosa, a gram-negative pathogen, were transferred to the containers and exposed to light (ie, a 60-watt domestic light bulb) for eight, 16, or 24 hours. The light intensity at the surface of the coating was measured at the start, in the middle, and at the end of each exposure period, and the effect of light on bacterial viability was determined. The Mann-Whitney U test was used to determine whether differences in the bacterial carrots were significant.