Lyme disease, potential plague of the 21st century detection problems resolved by imaging with the Bradford Variable Projection High Resolution Microscope

Townsend Letter for Doctors and Patients, Jan, 2005 by Robert W. Bradford, Henry W. Allen

Chart 4 Distinguishing Characteristics of Borrelia burgdorferi

Internal Flagella           Cyst Formation
Glycoprotein Coat           Destruction of B-Cells
DNA Net Arrangement         Camouflage as B-Cells
Bleb Formation              Internal Antigenic Proteins
Prolonged Replication Time  Surface Antigen Transformation
Cellular Invasion Ability   Spiral Shape

[c]2004 BRI

Chart 5 Some Lyme Disease Signs and Symptoms

Intense Fatigue                Burning/ Stabbing Pain
Memory Loss                    Tremors
Joint Pain/Swelling/Stiffness  Shortness of Breath
Poor Coordination              Anxiety
Slurred Speech                 Swollen Glands
Chills and/or Fever            Nausea/Vomiting
Rash                           Muscle Cramps
Sudden Mood Swings             Headaches/Migraines
Poor Concentration             Light Sensitivity
Unusual Depression             Tinnitus

[c]2004 BRI

Chart 6 Most Common Diseases Associated with Lyme

Alzheimer's Disease             Polymyalgia rheumatica
ALS                             Reflex sympathetic dystrophy
Bell's Palsy                    Rheumatoid Arthritis
Chronic Fatigue Syndrome (CFS)  Scleroderma
Fibromyalgia                    Syphilis
Irritable Bowel Syndrom         Multiple Sclerosis
Lupus                           Parkinson's Disease
Depression                      Autoimmune Disorders
Middle Ear Pressure             Tinnitus
Vertigo                         Rheumatoid Arthritis

[c]2004 BRI

Chart 7 Antibiotics Commonly Used in Lyme Treatment

Tetracycline     Salvarsan
Amoxacillin      Cefuroxime
Doxycycline      Clarithromycin
Flagyl           Metronidazole
Cefotaxime       Ceftriaxone
Azithromycin     Penicillin
Imipenem         Benzathine Penicillin
Cefdinir         Rocephin
Tinidazole       Trimethoprim
Cipro(floxacin)

[c]2004 BRI

Chart 9 Summary of Lyme Disease Therapy

Antibiotics: Includes Tetracycline, Amoxacillin, Cipro, Penicillin and
   Doxycycline
Oral Salt Therapy: THe enzyme elastase, found in neutrophils, is
   stimulated by high salt concentrations to remove a polypeptide LL-37
   from the precursor protein CAP-18. A group of the polypeptides
   assemble into a pore and becomes imbedded into the outer membrane of
   the infectious microorganism, allowing potassium and other ions to
   escape, thereby killing the organism.
Coloidal Silver: Small clusters of elemental silver atoms that bind to
   the spirochete, phagocytized by PMNs.
Bee Venom: Contains proteolytic enzymes that digest the outer coating of
   cysts. Also contains polypeptides that inhibit spirochete growth and
   reproduction.
Herbal Therapy: Includes fractionated Cat's Claw, an immune system
   stimulant and Artemesia, effective in combatting Babesia, a disease
   associated with Lyme.
Dioxychlor, Sulfoxime[TM], Bismacine-C[TM]: Bradford Research
   Institute/Ingles Hospital Therapy

[c]2004 BRI

Chart 10 -- Detection of Borrelia burgdorferi (Bb)

Fluorescent Antibody Test: Antibodies to Bb are covalently coupled to a
   fluorescent organic chemical and added to the patient's blood on a
   microscope slide. Antibodies bind to antigens found on Bb (spirochete
   or cyst form) and fluoresce under ultraviolet light, revealing the
   presence of Bb. Most accurate.
PCR: DNA from Bb is allowed to replicate, thereby increasing the amount
   present to enable a sequence determination to be made. The sequences
   are compared to the known sequences of Bb. Test unreliable.
ELISAL: Antibodies to Bb are covalently coupled to a specific enzyme and
   allowed to bind to Borrelia antigens in the presence of the enzyme
   substrate. Enzyme activity results in a color change, revealing the
   presence of Bb antigens.
Western Blot: The slab-gel electrophoresis of Lyme patient serum
   separates Bb protein antigens. A second transverse electrophoresis
   carries the antigens into a nitrocellulose membrane where they are
   revealed by the application of specific antibody and staining. Gross
   false-negatives.
Bradford High Resolution Microscopy: Both the cyst and spirochete forms
   in the three different morphologies are easily identified with
   resolutions less than 0.1 micron with concurrent magnification of
   10,000x utilizing dark-field and phase contrast modes.
Comparison of Tests: High Resolution Microscopy is the most reliable
   test. PCR, Western Blot and ELISA are the least reliable with up to
   80% false-negatives (CDC Guidelines).

[c]2004 BRI

Chart 11 -- Daily Intravenous Infusions

Infusion I (Nutrient, Antioxidant, Antimicrobial, 3-hour drip)
   The following in 250 cc Saline:
   10 cc DMSO
   25 g Vitamin C
   10 cc NAC
   10 cc Taurine Plus[TM]
   5 cc Biorizin[TM]
   2.5 cc Multivitamin Combination
   1 g EDTA
Infusion II in 100 cc saline, 1x/day, 10 cc Dioxychlor, over 30 minutes
Infusion III 2 cc each Bismacine-C[TM], Bismacine-N[TM], 2x/day, 1 hour
Infusion IV Sulfoxime[TM] (Antimicrobial) 1-3x/week, 200 cc, 20 min.
  drip
Infusion V Vitamin C 75 g in 250 cc saline, 2-hour drip, 1x/day

[c]2004 BRI

Chart 12 -- Daily Oral Program

   I. Vitamin C, 5 g 2x/day (controlled release)
  II. Inflazyme[TM], 5 tablets, 3x/day, 30 minutes before meals
 III. Oxy-5000[TM], 3 tablets, 3x/day
  IV. Magnesium Aspartate, 50 mg, 2x/day
   V. Calcium (Osteo Synergy[TM]), 50 mg, 2x/day
  VI. Potassium, 50 mg, 2x/day
 VII. Glutathione, 20 mg, 2-3x/day
VIII. Beta Carotene, 25,000 IU, 2x/day; Vitamin E, 400 IU, 3x/day;
      Vitamin B12 Compl., Caps., 1-3x/day; Selenium, 200 mcg,
      1-3x/day; Trace Minerals, 1-3x/day
  IX. Bowel Protocol (Overview)
      1. Ultra-Micro-Plex[TM], 4 tbs in 4 oz. water   pinch of salt,
         3x/day
      2. Rectal Implant, 1 tsp Ultra-Micro-Plex in 4 oz. salt water, 1-2
         x/day morning and night
      3. Coffee Enemas, 1x in morning
      4. Chamomile Enemas, 1x in afternoon
      5. HCI Protocol as Required

[c]2004 BRI

Chart 12A

    X. Liver Protector, Hepatrope, 1-3x/day
   XI. Glandular Support, Thymus 1-3x/day, Adrenal 1-3x/day, Thyroid,
       1-3x/day
  XII. Homeopathic Remedies as Indicated
 XIII. NeuroRecovery[TM], caps., 1-3x/day
  XIV. Oral Salt Treatment (Optional) 12 grams of Salt, spaced
       throughout the day
   XV. Artemesia
  XVI. Samento (Cat's Claw)
 XVII. Homeopathic Kidney Drainage
XVIII. Homeopathic Lymphatic Drainage
  XIX. Colloidal Silver

[c]2004 BRI