The ELISA guidebook; 2d ed

SciTech Book News, June, 2009

The ELISA guidebook; 2d ed.

Crowther, John R.

Humana Press Inc.

2009

566 pages

$129.00

Hardcover

Methods in molecular biology; v.516

QP519

Crowther (International Atomic Energy Agency, Animal Production and Health Section Vienna, Austria) presents an updated guide to the Enzyme Linked Immunosorbent Assay (ELISA), the predominant assay used in biomedical and agricultural research. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Little has changed in the principles of the ELISAs used since the publication of the first edition, but the second edition reflects technological advances since that time. The material has undergone some rearrangement for the new edition, and features three new chapters on charting methods for assessing the indirect ELISA, ruggedness and robustness of tests- aspects of kit use and validation, and internal quality control and external quality management of data.

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