Health Care Industry
Industry: Email Alert RSS FeedA review of automated slide stainers for IHC and ISH
Medical Laboratory Observer, Jan, 2008 by Joe Myers
Table 1. Primary attributes of available IHC/ISH slide-staining systems.
Slide
placement Operating
Vendor Product name format modes*
Biocare Medical intelliPath-FLX[TM] Array Open & closed
BioGenex i6000[TM] Array Open & closed
Laboratories
BioGenex xMatrx[TM] Array Open & closed
Laboratories
Dako Autostainer Universal[TM] Array Open & closed
(Thermo) Lab Vision Autostainer 360[TM] Array Open & closed
(Thermo) Lab Vision Autostainer 480[TM] Array Open & closed
(Thermo) Lab Vision Autostainer 720[TM] Array Open & closed
Ventana Med. Benchmark XT[TM] Carousel Closed
Systems
Ventana Med. Benchmark LT[TM] Carousel Closed
Systems
(Leica) Vision Bond[TM] Array Open / closed
Biosystems
Staining Reag. applic. Slide Reagent
Vendor capabilities** method capacity capacity
Biocare Medical IHC (ISH) Rinsed probe 50 48
BioGenex IHC (ISH) Pipette tips 60 60
Laboratories
BioGenex IHC, ISH Pipette tips 40 60
Laboratories
Dako IHC (ISH) Rinsed probe 48 64
(Thermo) Lab Vision IHC (ISH) Rinsed probe 36 40
(Thermo) Lab Vision IHC (ISH) Rinsed probe 48 40
(Thermo) Lab Vision IHC (ISH) Rinsed probe 72/84 84/40
Ventana Med. IHC, ISH Disposable 30 35
Systems dispensers
Ventana Med. IHC, ISH Disposable 20 25
Systems dispensers
(Leica) Vision IHC, ISH Rinsed probe 30 36
Biosystems
*Refers both to the ability of an instrument to incorporate reagents
from sources other than the manufacturer/distributor as well as the
ability to create user-defined staining protocols.
Table 2. Secondary attributes of available IHC/ISH slide-staining
systems.
Slide-label Reagent-label Adjustable
Product name scanning* scanning** dispensing
intelliPATH-FLX[TM] Yes Yes Yes
i6000[TM] Yes Yes Yes
xMatrx[TM] Yes Yes Yes
Autostainer Universal[TM] Yes Yes Yes
Autostainer 360[TM] Yes Yes Yes
Autostainer 480[TM] Yes Yes Yes
Autostainer 720[TM] Yes Yes Yes
Benchmark XT[TM] Yes Yes No
Benchmark LT[TM] Yes Yes No
Bond[TM] Yes Yes Yes
Slide heating Continuous
Product name Dewaxing (HIER/ISH) processing (^)
intelliPATH-FLX[TM] No No Yes
i6000[TM] Yes No Yes
xMatrx[TM] Yes Yes Yes
Autostainer Universal[TM] No No No
Autostainer 360[TM] No No No
Autostainer 480[TM] No No No
Autostainer 720[TM] No No No
Benchmark XT[TM] Yes Yes No
Benchmark LT[TM] Yes Yes No
Bond[TM] Yes Yes Yes
Multi-unit Reagent Waste
Product name control chilling (^^) separation
intelliPATH-FLX[TM] Yes Yes Yes
i6000[TM] No No No
xMatrx[TM] Yes No Yes
Autostainer Universal[TM] Yes No Yes
Autostainer 360[TM] Yes No Yes
Autostainer 480[TM] Yes No Yes
Autostainer 720[TM] Yes No Yes
Benchmark XT[TM] Yes No No
Benchmark LT[TM] Yes No No
Bond[TM] Yes No Yes
*Permits automatic staining protocol recognition.
**Permits automatic 'matching' of reagents with staining protocols and
relevant mismatch notification.
(^) Ability to continually add slides (or trays of slides) as
independent runs.
(^^) Refers to on-board refrigeration, used for reducing the rate of
reagent deterioration.
Table 3. Sample comsumables cost estimate.
Notes: 1) Slide-staining capacities specified below are based on
application of: 200[micro]L per slide
2) Discounted prices specified below are based on: 3% discount, as
provided for within vendor's Consumables Purchase Agreement
PREDILUTED ANTIBODIES:
Qty. Slides
Catalog Volume Slides per
Number Description (in ml) Stained Year
(Variable) Universal Negative Control 100 500 10,000
(Mouse/Rab.)
(Variable) Actin, Smooth-muscle -- Monocl. 6 30 1,000
[1A4]
(Variable) CD3 -- Monoclonal [PS1] 6 30 2,000
(Variable) CD20 -- Monoclonal [L26] 25 125 2,000
(Variable) Cytokeratin 7 -- Monoclonal 25 125 3,000
[K72.7]
(Variable) Cytokeratin 20 -- Monoclonal 25 125 3,000
[Ks20.8]
Vials 2007 Discounted
Catalog per Unit Price
Number Description Year Price per Unit
(Variable) Universal Negative Control 20 $202.00 $195.94
(Mouse/Rab.)
(Variable) Actin, Smooth-muscle -- Monocl. 34 $226.00 $219.22
[1A4]
(Variable) CD3 -- Monoclonal [PS1] 67 $166.00 $161.02
(Variable) CD20 -- Monoclonal [L26] 16 $548.00 $531.56
(Variable) Cytokeratin 7 -- Monoclonal 24 $755.00 $732.35
[K72.7]
(Variable) Cytokeratin 20 -- Monoclonal 24 $755.00 $732.35
[Ks20.8]
Extended/
Catalog Annualized Cost per
Number Description Cost Slide
(Variable) Universal Negative Control $3,918.80 $0.39
(Mouse/Rab.)
(Variable) Actin, Smooth-muscle -- Monocl. $7,453.48 $7.31
[1A4]
(Variable) CD3 -- Monoclonal [PS1] $10,788.34 $5.37
(Variable) CD20 -- Monoclonal [L26] $8,504.96 $4.25
(Variable) Cytokeratin 7 -- Monoclonal $17,576.40 $5.86
[K72.7]
(Variable) Cytokeratin 20 -- Monoclonal $17,576.40 $5.86
[Ks20.8]
Total Quantity of Slides Stained: 21,000
Total Annual Costs, Prediluted Antibodies: $65,818.38
Average Cost-per-Slide, Prediluted Antibodies: $4.84
DETECTION REAGENTS:
Qty. Slides
Catalog Volume Slides per
Number Description (in ml) Stained Year
(Variable) Universal Polymer-HRP 100 500 21,000
Detection Kit
(Variable) DAB-Chromogen/[H.sub.2] 110 550 21,000
[O.sub.2]-Substrate Kit
Units 2007 Discounted
Catalog per Unit Price
Number Description Year Price per Unit
(Variable) Universal Polymer-HRP 42 $1,065.00 $1,033.05
Detection Kit
(Variable) DAB-Chromogen/[H.sub.2] 39 $134.00 $129.98
[O.sub.2]-Substrate Kit
Extended/
Catalog Annualized Cost per
Number Description Cost Slide
(Variable) Universal Polymer-HRP $43,388.10 $2.07
Detection Kit
(Variable) DAB-Chromogen/[H.sub.2] $5,069.22 $0.24
[O.sub.2]-Substrate Kit
Total Annual Costs, Detection Reagents: $48,457.32
Cost-per-Slide, Detection Reagents: $2.31
ANCILLARY PRODUCTS:
Qty. Slides
Catalog Volume Slides per
Number Description (in ml) Stained Year
(Variable) Endogenous Peroxidase Blocking 500 2500 21,000
Agent
(Variable) TBS Wash Buffer, 20X 10,000 250 21,000
Concentrate
(Variable) Epitope Retrieval Solution, 5,000 500 20,000
10X (Conc.)
(Variable) Pepsin 25 120 5,000
(Variable) Slide Labels, Roll of 3200 N/A 3,200 25,000
(Variable) Vial Labels, Roll of 3000 N/A 3,000 3,000
Units 2007 Discounted
Catalog per Unit Price
Number Description Year Price per Unit
(Variable) Endogenous Peroxidase Blocking 9 $71.00 $68.87
Agent
(Variable) TBS Wash Buffer, 20X 84 $67.00 $64.99
Concentrate
(Variable) Epitope Retrieval Solution, 40 $228.00 $221.16
10X (Conc.)
(Variable) Pepsin 42 $59.00 $57.23
(Variable) Slide Labels, Roll of 3200 8 $342.00 $331.74
(Variable) Vial Labels, Roll of 3000 1 $342.00 $331.74
Extended/
Catalog Annualized Cost per
Number Description Cost Slide
(Variable) Endogenous Peroxidase Blocking $619.83 $0.03
Agent
(Variable) TBS Wash Buffer, 20X $5,459.16 $0.26
Concentrate
(Variable) Epitope Retrieval Solution, $8,846.40 $0.44
10X (Conc.)
(Variable) Pepsin $2,403.66 $0.48
(Variable) Slide Labels, Roll of 3200 $2,653.92 $0.10
(Variable) Vial Labels, Roll of 3000 $331.74 $0.11
Total Annual Costs, Ancillary Products: $20,314.71
Cost-per-Slide, Ancillary Products: $0.97
SUMMARY
Total Estimated Annual Costs: $134,590.41
Total Estimated Monthly Costs: $11,215.87
Overall Cost-per-Slide: $6.41
Most RecentHealth Care Articles
COPYRIGHT 2008 Nelson Publishing
COPYRIGHT 2008 Gale, Cengage Learning
- How to choose the right insurance carrier for your business
- Real Estate: Prepare your properties to weather what lies ahead
- Technology: Be prepared if part of your global supply chain goes missing
Most Recent Health Articles
Most Recent Health Publications
Most Popular Health Articles
- 50 home remedies that work: these safe, fast, and effective fixes will relieve what ails you - Cover Story
- Detox in 7 days: a detoux diet can help you shed up to 10 pounds and leave you feeling terrific. Our weeklong plan shows you how to lose the weight and keep it off - Cover story
- All about nightshades: explore the hidden hazards of your favorite food with macrobiotic nutritionist Lino Stanchich
- Treat sinusitis naturally: breath easy and relieve sinus pressure with these remedies - Quick Fixes and Long-Term Solutions
- La anemia falciforme - causas y tratamiento
Most Popular Health Publications
Content provided in partnership with
