A Comparison of Biosite Triage BNP to Roche Pro-BNP for Diagnosis of Heart Failure

Clinical Laboratory Science, Summer 2004 by Cabrera, Sandra, Quintana, Priscilla

ORAL PRESENTATIONS

Patients presenting to the hospital emergency room with chest pain and dyspnea must be quikly evaluated to differentiate pulmonary cause from heart failure. This study compared BType Natriuretic Peptide (BNP) with Pro-B-Type Natriuretic Peptide (Pro-BNP) to determine the best predictive indicator of heart failure and also to determine if renal disease caused any discrepancy in the diagnostic value of the tests. BNP was tested by Biosite's Triage fluorescence immunoassay and ProBNP by Roche's Elecsys System electrochemiluminescence immunoassay. One hundred and fifty hospital patient samples were tested by both methods. Test results agreed on 121 out of 150 samples based on patients' history, symptoms, and physician's diagnosis. Twenty nine patients were negative by Biosite BNP and positive by Roches Pro-BNP. Pro-BNP had 23 false positives, 13 of those had normal renal function. Biosite had no false positive and five false negative results. This suggests that Biosite is the more accurate method for this population. Further research must be done to investigate specific causes for the discrepancies.

Sandra Cabrera MS CLS(NCA), Texas A&M UniversityCorpus Christi, Corpus Christi TX; Priscilla Quintana MT(ASCP), Christus Spohn Shoreline Hospital, Corpus Christi TX.