Essential Oil Composition of Myrcia laruotteana Camb.

Abstract

Essential oils isolated by hydrodistillation from leaves and flowers of Myrcia laruotteana were analyzed by capillary GC and GC/MS. Twenty-five compounds, representing around 90% of the total oil, were identified. The main components were β-bisabolol (20.7 and 28.1, in leaves and flowers, respectively) and 14-hydroxy-α-muurolene (19.9% and 13.7%). Methyl salicylate was present only in flowers oil, as a minor constituent.

Key Word Index

Myrcia laruotteana, Myrtaceae, essential oil composition, α-bisabolol, 14-hidroxy-α-muurolene.

Plant Name

Myrcia laruotteana Camb., family Myrtaceae (1).

Source

Leaves and flowers were coUected in Curitiba, state of Paraná, southern region of Brazd, in October 2005. Voucher specimen was deposited at Herbarium of Universidade Federal do Paraná (UPCB).

Plant Part

The oils were obtained from the fresh leaves and flowers by hydrodistiUation for 2 h using a Clevenger-type apparatus and analyzed by GC and GC/MS. The yields (w/w) were 0.05% and 0.07% for leaves and flowers, respectively.

Previous Work

Essential oil of some Myrcia species has been reported (2^1), but chemical studies on Myrcia laruotteana were not found.

Present Work

The GC analysis was performed on a Shimadzu GC-17A gas Chromatograph with FID detector, in a split injector mode. A Durabond-DB5 capdlary column (30 m x 0.25 mm, 0.25 pm film thickness) was operated at 60°C for 3 min, and then programmed from 60°-220°C at 5°C/min, after which it was kept isothermal at 220°C for 5 min. The carrier gas was He ( 1 mL/min) and the injector temperature was 250°C. The relative percentage of individual components was based on the peak areas obtained by electronic integration without FID response factor correction.

The GC/EIMS (70 eV) analysis was performed on a Varian Saturn 2000 GC/ MS spectrometer equipped with a CP-Sd-8CB capdlary column (30 m x 0.25 mm, 0.25 pm film thickness) in the same conditions described above. The od components were identified by comparison of their retention indices (relative to n-alkanes) and mass spectra with those found in the literature (5) and stored on the spectrometer database (NIST 1998).

Altogether 25 compounds were identified, representing around 90% of die total oil. The components of the ods, the percentage of each constituent and the retention indices are summarized in Table I. It can be seen that M. laruotteana produces sesquiterpene rich ods, characterized by predominance of oxygenated sesquiterpenes (79.6% in leaves and 83.1% in flowers). Monoterpenes were present in small amounts, while methyl salicylate was found only in flowers oil as a minor constituent. The main components in both oils were α-bisabolol (20.7% in leaves and 28.1% in flowers) and 14-hydroxy-α-muurolene (19.9% and 13.7%).

References

1. CD. Legrand and R.M. Klein, Mirtáceas - Myrcia. In: Flora Ilustrada Catarlnense, Ed. R. Reitz, pp 218-326, Herbario Barbosa Rodrigues, Itajaí (1969).

2. R.P. Limberger, M. Sobral and A.T. Henriques, Óleos voláteis de espécies de Myrcia nativas do Rio Grande do Sul. Química Nova, 27, 916-919 (2004).

3. M.G.B. Zoghbi, E.H.A. Andrade, M.H.L da Silva, L.M.M. Carrelra and J.G.S. Maia, Essential oil from three Myrcia species. Flav. Fragr. J. 18, 421-424 (2003).

4. A. T. Henriques, M. Sobral, R. Bridi, P. Verin, C. Menut, G. Lamaty and J.M. Bessiere, Essential oils from five Southern Brazilian species of Myrcia (Myrtaceae). J. Essential Oil Res., 9, 13-18 (1997).

5. R.P. Adams, Identification of Essential Oil Components by Gas Chromatography/ Mass Spectroscopy. Allured Pubi. Corp., Carol Stream, IL (1995).

Maria Élida A Stefanello,*

Departamento de Química, Universidade Federal do Paraná, Cx. Postal 19081, 81531-990, Curitiba, PR, Brazil.

Armando C. Cervi,

Departamento de Botànica, Universidade Federal do Paraná, Caixa Postal 19041, 81531-990, Curitiba, PR, Brazil.

Alberto Wisniewski Jr and Edésio L. Simionatto,

Instituto de Pesquisas Tecnológicas de Blumenau, Universidade Regional de Blumenau, Rua Araçatuba 83, 89.030-080,

Blumenau, SC, Brazil.

"Address for correspondence

Received: March 2006

Accepted: May 2006

10412905/07/0005-0466$1 4.00/0- © 2007 Allured Publishing Corp.

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