Essential Oils of Two Varieties of Salvia euphratica Montbret et Aucher ex Benth. var. euphratica and var. leiocalycina (Rech. fil.) Hedge from Turkey, The

Abstract

Water distilled oils of two varieties of Salvia euphratica were analyzed by GC/MS. Ninety-five compounds in var. euphratica and 94 compounds in var. leiocalycina were characterized representing 93% and 95% of the total components detected, respectively, with 1,8-cineole (13.8% and 15.2%) and myrtenyl acetate (15.9% and 13.9%) as main constituents.

Key Word Index

Salvia euphratica, Labiatae, essential oil composition, 1,8-cineole, myrtenyl acetate.

Plant Name

Salvia euphratica Montbret et Aucher ex Benth. var. euphratica and Salvia euphratica Montbret et Aucher ex Benth. var. letocalycina (Rech, fil.) Hedge (1).

Source

Aerial parts of both varieties were collected in Malatya, Turkey in June 1999. Voucher specimens are kept at the Herbarium of the Faculty of Pharmacy, Anadolu University (ESSE: 13240, 13094).

Plant Part

Air-dried flowering aerial parts were hydrodistilled for 3 h using a Clevenger-type apparatus. The oil yields on moisture-free basis were 0.6% for both samples.

Previous Work

oil from aerial parts of S. euphratica var. euphratica obtained in 0.04% yield was shown to contain irans-pinocarvyl acetate (16.8%) and myrtenyl acetate (14.1%) as main constituents (2).

Present Work

The oils were analyzed by GC/MS using a Hewlett Packard GCD system. An HP-Innowax FSC column (60 m × 0.25 mm, 0.25 µm film thickness) was used with helium as carrier gas (1 mL/min). GC oven temperature was kept at 60°C for 10 min and programmed to 220°C at a rate of 4°C/min, kept constant at 220°C for 10 min and then programmed to 240°C at a rate of l°C/min. Split ratio was adjusted at 50:1. The injector temperature was at 250°C. MS were taken at 70 eV. Mass range was from 35 to 425 m/z. The components were identified by comparison of their mass spectra with Wiley GC/MS Library and Baser Library of Essential oil Constituents. Relative percentage amounts of the separated compounds were calculated automatically from peak areas of the total ion chromatogrammes. Alkanes were used as reference points in the calculation of relative retention indices (RRI). The compounds identified in the oil can be seen in Table I.

References

1. P.M. Davis, Flora of Turkey and the East Aegean Islands. Vol. 7, p. 431, University Press, Edinburgh (1982).

2. K.H.C. Baser, M. Kürkcüoglu and Z. Aytac, Composition of the essential oil of SaMa euphratica Montbret et Aucher ex Bentham var. euphratica from Turkey. Flav. Fragr. J., 13, 63-64 (1998).

K.H.C. Baser* and B. Demirci

Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey

B. Yildiz

Department of Biology, Faculty of Science and Letter, Balikesir University, 10100 Balikesir, Turkey

* Address for correspondence

Received: February 2002

Accepted: March 2002

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