Composition of the Essential Oil of Salvia limbata C.A. Mey

Abstract

The essential oil of Salvia limbata C.A. Mey isolated by steam distillation was analyzed by GC/MS. Among the 26 compounds identified the major components were germacrene D (25.7%), linalyl acetate (16.1%) and linalool (17.5%).

Key Word Index

Salvia limbata, Labiatae, essential oil composition, germacrene D, linalyl acetate, linalool.

Plant Name

Salvia limbata C.A. Mey. (syn. S. chrysadenia Freyn) (1-2).

Source

Aerial parts of the plant were collected from Tehran province (Damavand) in Iran at flowering stage in July 2001. A voucher specimen has been deposited at the Herbarium of TARI in Tehran, Iran.

Plant Part

The aerial parts of plant (250 g) were subjected to steam distillation in an all glass apparatus for l h to produce oil in 0.07% w/w yield.

Previous Study

A literature search reveals that the oil of Salvia limbata has not been the subject of previous study (2).

Present Work

Gas chromatography: GC analysis was performed using a Shimadzu GC-9A gas Chromatograph equipped with a DE-I fused silica column (60 m × 0.25 mm, film thickness 0.25 µm). Oven temperature was 50°C for 5 min and then programmed to 270°C at a rate of 4°C/min; injector and detector (FID) temperatures were 280°C; carrier gas was helium with a linear velocity of 32 cm/s. The relative amounts of individual components were based on electronic integration of peak area without the use of an internal standard or FID response factor correction.

GCMS: The oil of Salvia limbata was analyzed using a GC/MS (Varian 3400) equipped with a fused silica column (60 m × 0.25 mm, film thickness 0.25 µm) and interfaced with a Varian ion trap detector. Column temperature was programmed 50°-270°C at a rate of 4°C/min; injecter and transferline temperatures were 280°C and 290°C, respectively; the carrier gas was helium, which had a linear velocity of 31.5 cm/s split 1/60. For mass spectral measurement the following parameters were used: ionization energy 70 eV; scan time 1 s; mass range 40-300 amu. The identification of the constituents was assigned on the basis of mass spectra and retention indices, and wherever possible by co-injection with authentic standard compounds.

The compounds identified in the oil are listed in Table I.

Acknowledgement

The authors would like to acknowledge the financial support given by The Research Institute of Forests and Rangelands for this work.

References

1. V. Mozaffarian, A Dictionary of Iranian Plant Names, pp 522, Farhang Moaser, Tehran (1996).

2. J.D. Hooker and B.D. Jackson, Index Kewensis. Vo1 2. pp 1299. Clarendon Press Oxford (1960).

M. Mirza, V. Mozaffarian and Z. Baher Nik*

Research Institute of Forests and Rangelands, Department of Phy to chemistry, PO Box 13185-116, Tehran, Iran

* Address for correspondence

Received: August 2001

Revised: May 2002

Accepted: July 2002

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