Essential Oil of Pentapleura subulifera Hand.-Mazz., The

Journal of Essential Oil Research: JEOR, Mar/Apr 2005 by Baser, K H�sn� Can, Demirci, Bet�l, Tabanca, Nurhayat, Ayta�, Zeki, Ekici, Murat

Abstract

A water-distilled essential oil from the aerial parts of Pentapleura subulifera Hand.-Mazz., was analyzed by GC and GC/MS. Fifty-three components were characterized representing 95.9% of the oil with 1,8-cineole (39.0%) and T-cadinol (11.1%) as major constituents.

Key Word Index

Pentapleura subulifera, Labiatae, essential oil composition, 1,8-cineole, T-cadinol.

Plant Name

Pentapleura subulifera Hand.-Mazz. (1).

Source

Aerial parts of P. subulifera were collected in Sirnak: Senova in south eastern Turkey on July 15, 2001. Voucher specimens are kept at the Herbarium of Gazi University, Ankara, Turkey (ZA8175).

Plant Part

Air-dried flowering aerial parts were hydrodistilled for 3 h using a Clevenger-type apparatus. The yield of oil on moisture-free basis was 0.74%.

Previous Work

None.

Present Work

Gas chromatography: The oil was analyzed by GC using a Hewlett Packard 6890 system. An HP-Innowax FSC column (60 m x 0.25 mm, 0.25 �m film thickness) was used with nitrogen as the carrier gas (1 mL/min). The oven temperature was kept at 60�C for 10 min and programmed to 220�C at a rate of 4�C/min, then kept constant at 220�C for 10 min and then programmed to 240�C at a rate of 1�C/min. The injector temperature was at 250�C. The percentage compositions were obtained from electronic integration measurements using flame ionization detection (FID, 250�C). n-Alkanes were used as reference points in the calculation of relative retention indices (RRI). Relative percentages of the characterized components were area percents as cited in Table I.

GC/MS: The oil was analyzed by gas chromatography/mass spectrometry using a Hewlett Packard GCD system. Innowax FSC column (60 m x 0.25 mm, 0.25 �m film thickness) was used with helium as the carrier gas. GC oven temperature was kept at 60�C for 10 min and programmed to 220�C at a rate of 4�C/min, and then kept constant at 220�C for 10 min and programmed to 240�C at a rate of 1�C min. Split flow was adjusted at 50 mL/min. The injector temperature was at 250�C. MS were taken at 70 eV. Mass range was from m/z 35 to 425. Library search was carried out using Wiley GC/MS Library and Baser Library of Essential Oil Constituents.

References

1. P.H. Davis, Flora of Turkey and the East Aegean Islands. Vol. 7, p. 314, University Press, Edinburgh (1975).

K. H�sn� Can Baser,* Bet�l Demirci and Nurhayat Tabanca

Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey

Zeki Ayta� and Murat Ekici

Faculty of Science and Letters, Gazi University, 06500 Ankara, Turkey

* Address for correspondence

Received: May 2002

Accepted: July 2002

1041-2905/05/0002-0204$6.00/0-� 2005 Allured Publishing Corp.